Research Associate I – Molecular Biology
International Livestock Research Institute (ILRI) · Nairobi
Job description
About the role
The Research Associate I will support a multidisciplinary team in advancing plant genome editing and trait enhancement projects. The role involves construct development, molecular analyses, and maintenance of bacterial and plant cultures in a laboratory setting.
Key responsibilities
- Develop genome‑editing and RNAi constructs for target crops.
- Perform genomic DNA extraction from transgenic and gene‑edited events of date palm, sugarcane or other crops.
- Conduct PCR and quantitative real‑time PCR to assess gene expression and verify mutations.
- Execute mutation detection assays such as T7 endonuclease and band‑shift analysis.
- Carry out Southern blot analysis for copy‑number determination and transgene integration.
- Perform sequence analysis using Sanger or next‑generation sequencing to validate gene edits.
- Maintain and propagate bacterial cultures for plasmid DNA preparation.
- Support the upkeep of gene‑edited plant lines in the laboratory and greenhouse.
- Ensure accurate data collection, analysis, record‑keeping and assist in preparing reports, presentations and manuscripts.
- Collaborate with team members and provide technical support in molecular techniques.
Required profile
- Bachelor’s degree or diploma in molecular biology, biotechnology, genetics or a related field.
- Relevant professional certification.
- Two years of relevant experience with a degree (or four years with a diploma).
- Experience with in‑silico gene cloning tools such as Benchling and SnapGene.
- Hands‑on experience with DNA extraction, PCR, qRT‑PCR and sequencing.
- Familiarity with Southern blotting and mutation detection assays is a plus.
- Ability to maintain detailed lab records and follow protocols precisely.
- Strong organizational, time‑management, interpersonal and communication skills.
Required skills
- Genomic DNA extraction
- PCR and qRT‑PCR
- Southern blotting
- Mutation detection assays (T7 endonuclease, band‑shift)
- Sequencing (Sanger, NGS)
- Bacterial culture maintenance
- In‑silico cloning tools (Benchling, SnapGene)
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Published 3 days ago
Expires 3 weeks from now
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International Livestock Research Institute (ILRI)
Nairobi
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